pedf (R&D Systems)

R&D Systems pedf

Fig. 1 Characterisation of MSCs <t>and</t> <t>sEVs.</t> A Representative images of adipogenic (oil red O staining), osteogenic (alizarin red S staining), and chondrogenic (alcian blue staining) differentiation assay. B Analysis of sizes of sEVs from each group using Nanosight. C Representative transmission electron micrograph for each group; scale bar = 200 nm. D Representative western blots for loaded proteins and markers <t>(PEDF,</t> CD63, TSG101, and CD9) in MSC-sEVs. E Flow cytometry for measuring the loading efficiency of PEDF in PEDF-sEVs. FITC-labelled PEDF was used. F The concentrations of PEDF in sEVs and PEDF-sEVs were determined using ELISA (n = 3/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Pedf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+serpin+f1+pedf/Recombinant+Human+Serpin+F1%2FPEDF+Protein%2C+CF/pm37684667-75-0-3
Average 93 stars, based on 1 article reviews

pedf - by Bioz Stars, 2026-09

93/100 stars

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recombinant human serpin f1 pedf protein (Novus Biologicals)

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The Recombinant Human Serpin F1 PEDF Protein from Novus Biologicals is derived from E coli The Recombinant Human Serpin F1 PEDF Protein has been validated for the following applications SDS Page

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recombinant human serpin f1 pedf protein cf (R&D Systems)

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The Recombinant Human Serpin F1 PEDF Protein from R D Systems is derived from NS0 The Recombinant Human Serpin F1 PEDF Protein has been validated for the following applications Bioactivity

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recombinant human serpin f1 (pedf) (carrier-free) (BioLegend)

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Recombinant Human Serpin F1 (PEDF) (carrier-free) Apps: BA; Size: 10 µg

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recombinant human serpin f1/pedf protein (Novus Biologicals)

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Recombinant Human Serpin F1/PEDF Protein

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recombinant human serpin f1/pedf protein (Aladdin Scientific Corporation)

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Purity＞97% by SDS-PAGE and HPLC analyses.FunctionPigment epithelium-derived factor (PEDF) is encoded by the SERPINF1 gene in humans and found in verebrates. It is a secreted phosphoglycoprotein that belongs to the clade F subfamily, serpin superfamily

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recombinant human serpin f1/pedf protein, cf (R&D Systems)

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Recombinant Human Serpin F1/PEDF Protein, CF

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Fig. 1 Characterisation of MSCs and sEVs. A Representative images of adipogenic (oil red O staining), osteogenic (alizarin red S staining), and chondrogenic (alcian blue staining) differentiation assay. B Analysis of sizes of sEVs from each group using Nanosight. C Representative transmission electron micrograph for each group; scale bar = 200 nm. D Representative western blots for loaded proteins and markers (PEDF, CD63, TSG101, and CD9) in MSC-sEVs. E Flow cytometry for measuring the loading efficiency of PEDF in PEDF-sEVs. FITC-labelled PEDF was used. F The concentrations of PEDF in sEVs and PEDF-sEVs were determined using ELISA (n = 3/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Journal: Journal of nanobiotechnology

Article Title: Enhanced therapeutic effect of PEDF-loaded mesenchymal stem cell-derived small extracellular vesicles against oxygen-induced retinopathy through increased stability and penetrability of PEDF.

doi: 10.1186/s12951-023-02066-z

Figure Lengend Snippet: Fig. 1 Characterisation of MSCs and sEVs. A Representative images of adipogenic (oil red O staining), osteogenic (alizarin red S staining), and chondrogenic (alcian blue staining) differentiation assay. B Analysis of sizes of sEVs from each group using Nanosight. C Representative transmission electron micrograph for each group; scale bar = 200 nm. D Representative western blots for loaded proteins and markers (PEDF, CD63, TSG101, and CD9) in MSC-sEVs. E Flow cytometry for measuring the loading efficiency of PEDF in PEDF-sEVs. FITC-labelled PEDF was used. F The concentrations of PEDF in sEVs and PEDF-sEVs were determined using ELISA (n = 3/group). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: PEDF (1177- SF-025, R&D SYSTEMS, USA) was mixed with sEVs at a 1:5 concentration ratio.

Techniques: Staining, Differentiation Assay, Transmission Assay, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Fig. 3 PEDF-sEVs suppress the expression of inflammatory cytokines in HRECs. A Starved HRECs were pre-treated with PEDF, sEVs, or PEDF-sEVs under stimulation with 10 ng/mL VEGF for 24 h. Representative western blot images showing the expression of ICAM-1 in HRECs. B Relative expression of ICAM-1 compared with that of GAPDH (n = 3). C, D, E. PCR analysis of the expression of TNF-α, VCAM-1, and IL-1β in HRECs (n = 5). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Journal: Journal of nanobiotechnology

doi: 10.1186/s12951-023-02066-z

Figure Lengend Snippet: Fig. 3 PEDF-sEVs suppress the expression of inflammatory cytokines in HRECs. A Starved HRECs were pre-treated with PEDF, sEVs, or PEDF-sEVs under stimulation with 10 ng/mL VEGF for 24 h. Representative western blot images showing the expression of ICAM-1 in HRECs. B Relative expression of ICAM-1 compared with that of GAPDH (n = 3). C, D, E. PCR analysis of the expression of TNF-α, VCAM-1, and IL-1β in HRECs (n = 5). The data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

Article Snippet: PEDF (1177- SF-025, R&D SYSTEMS, USA) was mixed with sEVs at a 1:5 concentration ratio.

Techniques: Expressing, Western Blot

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